Linkage analysis of er-1 , a recessive Pisum sativum gene for resistance to powdery mildew fungus ( Erysiphe pisi D.C.)
Identifieur interne : 004425 ( Main/Exploration ); précédent : 004424; suivant : 004426Linkage analysis of er-1 , a recessive Pisum sativum gene for resistance to powdery mildew fungus ( Erysiphe pisi D.C.)
Auteurs : G. M. Timmerman [Nouvelle-Zélande] ; T. J. Frew [Nouvelle-Zélande] ; N. F. Weeden [États-Unis] ; A. L. Miller [Nouvelle-Zélande] ; D. S. Goulden [Nouvelle-Zélande]Source :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 1994-09-01.
English descriptors
- KwdEn :
- Teeft :
- Almota, Amplification, Best order, Cold spring harbor, Cold spring harbor laboratory press, Crop food research, Different gene, Distinct genes, Dominant marker, Downy mildew resistance genes, Genetic linkage, Genetic maps, Harland, Heringa, Joint segregation analyses, Joint segregation analysis, Kosambi function, Large number, Linkage, Linkage group, Linkage mapping, Linkage maps, Locus, Many markers, Marker, Mildew, Molecular markers, Mosaic virus, Other linkage groups, Phenotypic class, Pisum, Pisum sativum, Powdery, Powdery mildew, Powdery mildew fungus, Powdery mildew resistance, Powdery mildews, Precise order, Primer, Proc natl acad, Progeny, Rapd, Rapd amplifications, Rapd marker, Rapd product, Rapd products, Recombinant inbred lines, Recombination fractions, Repulsion phase, Resistant plants, Restriction fragment length polymorphism, Rils, Sativum, Segregant, Segregant analysis, Segregation patterns, Specific primer pair, Susceptible plants, Tight linkage, Timmerman, Weeden, Whitehead institute, Wolko.
Abstract
Abstract: Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR).
Url:
DOI: 10.1007/BF00220815
Affiliations:
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Goulden</name><affiliation wicri:level="1"><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch</wicri:regionArea><wicri:noRegion>Christchurch</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Theoretical and Applied Genetics</title><title level="j" type="sub">International Journal of Plant Breeding Research</title><title level="j" type="abbrev">Theoret. Appl. Genetics</title><idno type="ISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1994-09-01">1994-09-01</date><biblScope unit="volume">88</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1050">1050</biblScope><biblScope unit="page" to="1055">1055</biblScope></imprint><idno type="ISSN">0040-5752</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bulked segregant analysis</term><term>Genome mapping</term><term>Molecular markers</term><term>Pea</term><term>Powdery mildew</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Almota</term><term>Amplification</term><term>Best order</term><term>Cold spring harbor</term><term>Cold spring harbor laboratory press</term><term>Crop food research</term><term>Different gene</term><term>Distinct genes</term><term>Dominant marker</term><term>Downy mildew resistance genes</term><term>Genetic linkage</term><term>Genetic maps</term><term>Harland</term><term>Heringa</term><term>Joint segregation analyses</term><term>Joint segregation analysis</term><term>Kosambi function</term><term>Large number</term><term>Linkage</term><term>Linkage group</term><term>Linkage mapping</term><term>Linkage maps</term><term>Locus</term><term>Many markers</term><term>Marker</term><term>Mildew</term><term>Molecular markers</term><term>Mosaic virus</term><term>Other linkage groups</term><term>Phenotypic class</term><term>Pisum</term><term>Pisum sativum</term><term>Powdery</term><term>Powdery mildew</term><term>Powdery mildew fungus</term><term>Powdery mildew resistance</term><term>Powdery mildews</term><term>Precise order</term><term>Primer</term><term>Proc natl acad</term><term>Progeny</term><term>Rapd</term><term>Rapd amplifications</term><term>Rapd marker</term><term>Rapd product</term><term>Rapd products</term><term>Recombinant inbred lines</term><term>Recombination fractions</term><term>Repulsion phase</term><term>Resistant plants</term><term>Restriction fragment length polymorphism</term><term>Rils</term><term>Sativum</term><term>Segregant</term><term>Segregant analysis</term><term>Segregation patterns</term><term>Specific primer pair</term><term>Susceptible plants</term><term>Tight linkage</term><term>Timmerman</term><term>Weeden</term><term>Whitehead institute</term><term>Wolko</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR).</div></front></TEI><affiliations><list><country><li>Nouvelle-Zélande</li><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><country name="Nouvelle-Zélande"><noRegion><name sortKey="Timmerman, G M" sort="Timmerman, G M" uniqKey="Timmerman G" first="G. M." last="Timmerman">G. M. Timmerman</name></noRegion><name sortKey="Frew, T J" sort="Frew, T J" uniqKey="Frew T" first="T. J." last="Frew">T. J. Frew</name><name sortKey="Goulden, D S" sort="Goulden, D S" uniqKey="Goulden D" first="D. S." last="Goulden">D. S. Goulden</name><name sortKey="Miller, A L" sort="Miller, A L" uniqKey="Miller A" first="A. L." last="Miller">A. L. Miller</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Weeden, N F" sort="Weeden, N F" uniqKey="Weeden N" first="N. F." last="Weeden">N. F. Weeden</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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